A Comparative Evaluation of Antimicrobial and Five Physical Properties of Irreversible Hydrocolloid Incorporated with Silver Nanoparticles and Curcumin: An In-Vitro Study

Abstract

 Background and Objectives: Dental impressions are widely used in dentistry and they come in contact with patient's saliva, blood and bacterial plaque. Therefore, disinfection is mandatory. The purpose of this study is to evaluate and compare antimicrobial property of irreversible hydrocolloid impression materials incorporated with silver nanoparticles and curcumin and its effect on ve physical properties- gelation time, gel strength, permanent deformation, ow and dimensional accuracy. Materials and Methods: A total of 64 specimens, 32 for each brand and subgroups used (Zelgan and Tropicalgin) were mixed according to manufacturer's instruction. Silver nanoparticles and curcumin were added as antimicrobial agents. The samples were tested for antimicrobial activity against Staphylococcus aureus and Candida albicans and for ve physical properties - gel strength, permanent deformation, ow, gelation time and dimensional accuracy. Statistical analysis was done with one-way ANOVA to compare within the group and Tukey post hoc to compare between the groups. Results: The results of silver nanoparticles in Zelagan irreversible hydrocolloid showed increase in antimicrobial activity against C. albicans(7mm) and S. aureus(17mm). The mean gelation time(126sec), permanent deformation(43.2%) increased whereas, and gel strength(1.4MPa) decreased and curcumin in Zelagan irreversible hydrocolloid showed increase in antimicrobial activity against C. albicans(18mm) and S. aureus(27mm). The mean gelation time(105sec) and gel strength(4.3MPa) and ow(17mm) increased whereas, permanent deformation(24%) decreased. Silver nanoparticles in Tropicalgin irreversible hydrocolloid showed increase in antimicrobial activity against C. albicans(15mm) and S. aureus(15mm). The mean gelation time(40sec), permanent deformation(28.6%), gel strength(5MPa) and ow(17sec) increased and curcumin in Tropicalgin irreversible hydrocolloid showed increase in antimicrobial activity against C. albicans(7mm) and S. aureus(22mm). The mean gel strength(4.9MPa) and ow(17.4mm) increased whereas, permanent deformation(24.6%) and gelation time(94sec). Interpretation and Conclusion: From this invitro study, we conclude that, the incorporation of silver nanoparticles and curcumin in irreversible hydrocolloid (Zelgan and Tropicalgin) showed statistically signicant improvement in antimicrobial activity and all the physical properties. Zelgan incorporated with curcumin was the best combination.